GynaecologyFree in the app
Subfertility — first investigations
No conception after 12 months of regular sex.
Source: NICE NG257 (2026)Tests
- Mid-luteal (day 21 of 28) progesterone to confirm ovulation
- Semen analysis (repeat at 3 months if abnormal)
- Tubal patency: HSG/HyCoSy; laparoscopy & dye if PID/endometriosis history
- AMH/AFC only to predict IVF response
Semen (WHO lower limits)
| Volume | ≥1.4 ml |
|---|---|
| Concentration | ≥16 million/ml |
| Total motility | ≥42% |
| Progressive | ≥30% |
| Normal forms | ≥4% |
A summary for trained clinicians. Always check the full guideline, the patient in front of you and your hospital's protocol. Spotted an error? Tell us.